质粒名称: | pEGFP-NMHC II-C |
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出品公司: | 爱迪基因 |
目录编号: | 10843 |
存储实验室: | Robert Adelstein |
载体骨架基本信息 |
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载体名称: | pEGFP-N3 |
载体出品公司: | Clontech |
载体抗性: | 卡那霉素 |
空载体大小: | 4700 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | 新霉素(G418) |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | NMHC II-C,nonmuscle myosin II-C heavy chain |
物种来源: | M. musculus (mouse) |
插入大小: | 6400 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | EGFP(主干上的C端) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | SalI(未破坏) |
3' 克隆位点: | BamHI(未破坏) |
5' 测序引物 | EGFP-N |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pEGFP-NMHC II-C was a gift from Robert Adelstein (Addgene plasmid # 10843 ; http://n2t.net/addgene:10843 ; RRID:Addgene_10843) |
参考文献部分: | Identification and characterization of nonmuscle myosin II-C, a new member of the myosin II family. Golomb E, Ma X, Jana SS, Preston YA, Kawamoto S, Shoham NG, Goldin E, Conti MA, Sellers JR, Adelstein RS. J Biol Chem. 2004 Jan 23. 279(4):2800-8. 10.1074/jbc.M309981200 PubMed 14594953 |
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载体基本信息
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载体简介
载体序列
LOCUS pEGFP-NMHC II-C 10708 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pEGFP-NMHC II-C SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10708) AUTHORS Golomb E, Ma X, Jana SS, Preston YA, Kawamoto S, Shoham NG, Goldin E, Conti MA, Sellers JR, Adelstein RS TITLE Identification and characterization of nonmuscle myosin II-C, a new member of the myosin II family. JOURNAL J Biol Chem. 2004 Jan 23. 279(4):2800-8. PUBMED 14594953 REFERENCE 2 (bases 1 to 10708) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10708 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 68..371 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 372..575 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 526..546 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 664..673 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 6655..6664 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6661..7380 /codon_start=1 /product="enhanced GFP" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6706..6727) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6967..6986) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 7314..7335 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 7503..7624 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7540..7559) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7594..7613 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(7631..8086) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7768..7789) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 7980..7999 /label=F1ori-R /note="F1 origin, reverse primer" promoter 8113..8217 /gene="bla" /label=AmpR promoter promoter 8219..8576 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(8242..8262) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 8427..8562 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8489..8508 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 8611..9405 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(8665..8684) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 9275..9294 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(9593..9612) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 9637..9684 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 10013..10601 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10502..10521 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtatcgat 661 gccgccacca tggctgcagt gaccatgtcc gtgtctggga ggaaggtagc ctccaggcca 721 ggcccggtgc ctgaggcagc ccaatcgttc ctctacgcgc cccggacgcc aaatgtaggt 781 ggccctggag ggccacaggt ggagtggaca gcccggcgca tggtgtgggt gccctcggaa 841 ctgcatgggt tcgaggcagc agccctgcgg gatgaagggg aggaggaggc agaagtggag 901 ctggcggaga gtgggcgccg cctgcggctg cccagggacc agatccagcg catgaaccca 961 cccaagttca gcaaggcaga agatatggct gagctcacct gcctcaacga ggcctcggtc 1021 ctgcacaacc tgcgagaacg ctactactcc gggctcattt atacctactc tggcctcttc 1081 tgtgtggtca ttaacccata caagcagctg cccatctaca cggaggccat tgttgaaatg 1141 taccggggca agaagcgcca tgaggtgcca cctcacgtgt atgctgtgac ggagggcgcg 1201 taccgcagca tgcttcagga tcgtgaggat caatccattc tctgcacggg agagtctggc 1261 gctgggaaga cggagaacac caagaaggtc atccagtacc tggcccatgt ggcatcatct 1321 ccaaagggca ggaaggagcc tggtgtccct ggggagctag agcgtcagct tcttcaagcc 1381 aaccccatcc tagaggcctt tggcaatgcc aagacagtga agaacgacaa ctcttcccga 1441 tttggcaaat tcatccgcat caactttgat attgctggct acatcgtggg agcaaacatc 1501 gagacctatc tgttggagaa gtcccgggcc atcagacagg ccaaggatga atgcagcttc 1561 catatcttct accagctgct agggggcgct ggggagcagc taaaagctga cctccttctg 1621 gagccctgtt cccattatcg cttcctgacc aatgggccct catcgtcccc gggccaggag 1681 cgtgagttat tccaggagac cctggagtcc ctgcgtgtgc tgggcctcct cccagaagag 1741 atcactgcca tgctgcgcac tgtctctgct gtcctccagt ttggcaacat tgtcctgaag 1801 aaagagcgca atacggacca agccaccatg cctgacaaca cagctgccca gaagctttgc 1861 cgcctcttgg gactcggagt gaccgacttc tccagagccc ttctcacacc ccgcatcaaa 1921 gtgggccgag attatgttca gaaagcacaa accaaggagc aggctgactt tgcgctggag 1981 gctctggcca aagctaccta tgagcgcctg ttccgctggc tggttctgcg gctcaaccgt 2041 gccctggaca gaagcccgcg gcagggtgcc tccttcctgg gcatcctgga catcgcgggc 2101 tttgagatct tccagctgaa ctccttcgag cagctgtgca tcaactacac caacgagaag 2161 ctacagcagc tattcaacca caccatgttc gtgctggagc aggaggagta ccagcgagag 2221 ggcatcccct ggaccttcct agacttcggg ttggacctgc aaccttgcat cgacctcatt 2281 gagcgtccgg ccaaccctcc aggtctcctg gccctgctgg acgaggagtg ctggttcccc 2341 aaggccacgg acaagtcttt tgtggagaag gtcgcccagg agcagggcag ccaccccaaa 2401 ttccagcgcc ccaggaacct gcgagatcag gccgacttca gcgtcctgca ctacgccggc 2461 aaggttgact acaaagccag tgagtggctg atgaagaaca tggacccact gaatgacaat 2521 gtggccgcct tgcttcacca gagcacggat cgtctcacag ctgagatctg gaaggatgtg 2581 gagggcatcg tggggctgga gcaagtaagc agccttggag atggcccacc gggaggccgc 2641 ccccgccgtg gaatgttccg gactgtgggg cagctctaca aagaatccct gagccgcctc 2701 atggccacgc tcagcaacac caaccctagt tttgtccgct gcatcgttcc caatcatgag 2761 aagagggctg gaaagctgga gccgcgcctg gtgctggacc aactccgttg taacggggtc 2821 ctcgagggta tacgcatctg tcgccaaggc ttccccaacc gcatcctctt ccaggagttc 2881 cgacagcgct atgaaatcct caccccgaac gctattccca agggcttcat ggacggcaaa 2941 caggcctgtg agaagatgat ccaggccctg gagctagacc ccaacctgta ccgtgttggc 3001 caaagcaaga tcttcttccg ggcaggggtc ctggcccagc tggaggagga gcgggacctg 3061 aaagtcaccg acatcatagt gtctttccag gcagcggcac ggggctacct ggcccgtagg 3121 gctttccaga gacggcagca gcagcagagt gctctgaggg tgatgcagag aaactgtgct 3181 gcctacctca agctcaggaa ctggcagtgg tggaggctgt tcatcaaggt gaagcccctg 3241 ctgcaggtga cacggcagga tgaggtgctg caggcgcgcg cccaggagct gcagaaagtt 3301 caggagctgc agcagcagag cgctcgtgaa gtgggggaac tgcagggtcg agtggcacag 3361 ctagaggagg agcgcacgcg cctggctgag cagcttcgag cagaagccga gctctgctct 3421 gaggccgagg agacgcgggc gcgactggct gcccggaagc aggagctgga gctggtggtg 3481 acagagctgg aggcacgagt gggcgaggaa gaagagtgca gccggcagct gcagagtgag 3541 aagaagaggc tgcagcagca tatccaggag ctagagagcc acctggaagc tgaggagggt 3601 gcccggcaga agctacagct ggagaaggtg accacagagg ccaagatgaa gaaatttgag 3661 gaggacctgc tgctcctgga ggaccagaat tccaagctga gcaaggagcg gaggctgctg 3721 gaggagcggc tggctgagtt ctcctcacag gcagcagaag aggaagagaa agtcaaaagt 3781 ctcaacaagc tgaggctcaa atatgaagcc acaatctcag acatggaaga ccggctgaag 3841 aaggaggaga agggacgcca ggaactagag aagctgaagc gacggctgga cggggagagc 3901 tcagagcttc aggagcagat ggtggagcag aagcagaggg cagaggaact gctcgcacag 3961 ctgggccgca aggaggatga gctgcaggcc gccctgctca gggcagagga agagggtggt 4021 gcccgtgccc agttgctcaa gtccctgcga gaggcacagg ctggccttgc tgaggctcag 4081 gaggacctgg aagctgagcg ggtagccagg gccaaggcgg agaagcagcg ccgggacctg 4141 ggcgaggagt tggaggccct acgtggggag ctcgaggaca ctctggattc caccaacgcc 4201 cagcaggagc tgcggtccaa gagggagcag gaggtgacag agctgaagaa agcattggaa 4261 gaggagtccc gtgcccatga ggtgtccatg caggagctga gacagaggca tagccaggca 4321 ctggtggaga tggccgagca gttggagcaa gcccggaggg gcaaaggtgt gtgggagaag 4381 actcggctat ccctggaggc tgaggtgtcc gagctgaagg ccgagctgag cagcctgcag 4441 acctcgagac aggagggtga gcagaagagg cgccgcctgg agtcccagct acaggaggtc 4501 cagggccgat ccagtgattc ggagcgggct cggtctgagg ctgctgagaa gctgcagaga 4561 gcccaggcgg aacttgagag cgtgtccaca gccctgagtg aggcggagtc caaagccatc 4621 aggctgggca aggagctgag cagtgcagag tcccagctgc atgacaccca ggaactgctt 4681 caggaggaga ccagggcaaa gctggccttg gggtcccgtg tgcgtgccct agaggccgag 4741 gcggcggggc ttcgggagca gatggaagag gaggtggttg ccagggaacg ggctggccgg 4801 gagctgcaga gcacgcaggc ccagctctct gaatggcggc gccgccagga agaagaggct 4861 gcggtgctgg aggctgggga ggaggctcgg cgccgtgcag cccgggaggc agagaccctg 4921 acccagcgcc tggcagaaaa gactgaggct gtagaacgac tggagcgagc ccggcgccga 4981 ctgcagcagg agttggacga tgccactgtg gatctggggc agcagaagca gctcctgagc 5041 acactggaga agaagcagcg gaaatttgac cagctcctgg cagaggagaa ggctgcagtt 5101 ctacgggctg tggaagaccg tgaacggata gaggccgaag gccgggagcg agaggcccgg 5161 gccctgtcgc tgacccgggc cctggaagag gagcaggagg cccgggagga gctggagagg 5221 cagaaccgtg ctctgagggc tgagctggaa gcactgctga gcagcaagga tgacgtgggc 5281 aagaacgtgc acgagctgga gcgagcccgt aaggcggctg aacaggcagc cagtgacctg 5341 cggacacagg tgacagaatt ggaggatgag ctgacagccg cagaggatgc caagctgcgc 5401 ctggaggtga ctgtgcaggc tctgaaggct caacatgaac gcgacctgca gggccgcgat 5461 gatgccggtg aggagaggcg gaggcagctg gccaagcagc taagagacgc agaggtagag 5521 cgcgatgagg aacggaagca gagggcactg gctatggctg cccgcaagaa gctggagctg 5581 gaactggagg agttgaaggc gcagacatct gctgctgggc agggcaagga agaggcagtg 5641 aagcagctga agaagatgca ggtccagatg aaggagctgt ggcgggaggt agaggagacg 5701 cgtagctccc gcgacgagat gtttaccctg agcagggaaa atgagaagaa gctcaagggg 5761 ctggaagctg aggtgctgcg tctgcaagag gaacttgctg cctcagaccg agcccggagg 5821 caggcccagc aagacagaga cgagatggca gaggaggtgg ccagtggcaa tcttagcaag 5881 gcagccaccc tggaggaaaa acggcagctg gaggggcgac tgagccagtt ggaagaggag 5941 ctggaggaag aacagaacaa ctcggagctg ctcaaggacc attaccgaaa gctagtgcta 6001 caggtcgagt ccctcaccac agaactgtct gccgaacgaa gtttctcagc caaggccgag 6061 agtggacggc agcagctgga gcggcagatc caggaactgc gggcccgctt gggtgaagag 6121 gatgctggag cccgagccag gcagaaaatg ctgatcgctg ctctggagtc taaactggcc 6181 caggcagagg agcagctgga gcaggagagc agggagcgca tcctctctgg caagctggta 6241 cgcagagctg agaagcggct gaaggaggta gttcttcagg tggatgaaga gcgcagggtg 6301 gctgaccagg tccgggacca gctggagaaa agcaacctcc ggctgaagca gctcaagagg 6361 cagctggagg aggcagagga ggaggcatct cgggcacagg ctggtcggag gcggctgcag 6421 cgggagctgg aggacgtcac tgagtctgca gaatccatga accgggaggt gaccacgctg 6481 aggaacaggc tccggcgtgg cccacttaca ttcaccacac ggactgtgcg ccaggtgttc 6541 cggctggaag agggcgtggc ttctgacgag gaagaggctg aaggagctga acctggctct 6601 gcaccaggcc aggagccgga ggctccgccc cctgccacac cccaaggatc catcgccacc 6661 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 6721 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 6781 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 6841 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 6901 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 6961 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 7021 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 7081 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 7141 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 7201 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 7261 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 7321 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa 7381 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt 7441 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg 7501 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 7561 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 7621 cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa 7681 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa 7741 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 7801 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 7861 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct 7921 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 7981 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 8041 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt 8101 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 8161 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct 8221 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 8281 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa 8341 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 8401 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 8461 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 8521 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc 8581 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt 8641 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 8701 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 8761 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg 8821 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 8881 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 8941 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 9001 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 9061 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 9121 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 9181 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 9241 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 9301 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 9361 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt 9421 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg 9481 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 9541 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac 9601 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa 9661 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg 9721 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac 9781 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc 9841 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat 9901 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 9961 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 10021 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 10081 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 10141 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 10201 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 10261 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 10321 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 10381 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 10441 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 10501 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 10561 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 10621 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 10681 ctgattctgt ggataaccgt attaccgc //
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